Potential transformation of seagrass (Syringodium isoetifolium) into a bioactive food ingredient using different extraction techniques

Background: Syringodium isoetifolium is a seagrass that grows abundantly in Indonesian territorial waters and has been known to be of high significance not only for the seawater ecosystem, but also human beings (as food, nutritional pharmaceutical products). In this study, bioactive constituent was extracted using several different techniques recover maximum yield. Methods: Extraction was carried out by conventional non-conventional (Microwave-assisted extraction, ultrasound-assisted extraction-bath system, extraction- (UAE) probe system) with green solvents (water, 50% ethanol, 100% ethanol). Results: As result, ethanol water extracts exhibited significantly higher yield. Total phenol content extract. Different extraction (using solvent) showed UAE-probe best technique since it yielded highest total (17.37 ± 2.16 mg GAE/g) richest compounds (Choline, betaine, 3,5-di-tert-butyl-4-hydroxybenzoic acid, 7-Hydroxycoumarine, 4-Methoxycinnamic Zearalenone, Caffeic Levalbuterol, Phloretin, Dihydrocaffeic Quercetin-3β-D-glucoside Quercetin). Interestingly, choline most abundant compound extract obtained techniques. in silico assay, from shown as an anti-inflammatory. The interaction pathway receptors (TNF-α, IL-1β, IL6) had binding affinity value than inhibitor-receptor (i.e. -3.4, -3.0, -2.8 kcal/mol). cytotoxicity test on TIG-1 cells did have toxic effect them. Conclusions: These findings support potential use as food ingredient.